Thioredoxin 1, Recombinant, Human (Thioredoxin-1, Trx-1, trxA, fipA, tsnC, b3781, JW5856, Thioredoxin 1)
Catalog No : USB-T1010-01M
456.33€
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| Product name | Thioredoxin 1, Recombinant, Human (Thioredoxin-1, Trx-1, trxA, fipA, tsnC, b3781, JW5856, Thioredoxin 1) | ||
|---|---|---|---|
| Catalog No | USB-T1010-01M | ||
| Supplier’s Catalog No | T1010-01M | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E.coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 11.7 | ||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDS-PAGE) | ||
| Form | Supplied as a lyophilized powder from 20mM HEPES | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDS-PAGE) | ||
| Description | Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide -isomerase (PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins. Source: Recombinant protein corresponding to aa1-105, from human Thioredoxin 1 expressed in E.coli. Molecular Weight: ~11.7kD Specific Activity: ~10 U/mg (One unit will cause a change of A650 of 1 in 1 min at 25°C in the insulin reduction assay) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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