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Categories: Peptides & Proteins / Proteins

Product name			Thioredoxin 1, Recombinant, E. coli, aa2-109, His-Tag (DasC, FipA, TsnC)
Catalog No			USB-170086
Supplier’s Catalog No			170086
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			12.9

Storage			-20°C
Other names
Grade			Purified
Purity			~85% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol.
Reactivity life			12 months
Note			For reserch purpose only
Purity			~85% (SDS-PAGE)
Description			Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. It has also recently been found to play a role in cell-to-cell communication. Source: Recombinant protein corresponding to aa2-109 from E. coli Thioredoxin 1, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~12.9kD (119aa, aa2-109 NT + His Tag) Biological Activity: Specific activity is 16-18 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin. AA Sequence: MHHHHHHMGS DKIIHLTDDS FDTDVLKADG AILVDFWAEW CGPCKMIAPI LDEIADEYQG KLTVAKLNID QNPGTAPKYG IRGIPTLLLF KNGEVAATKV GALSKGQLKE FLDANLAGS Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.