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Categories: Peptides & Proteins / Proteins

Product name			Disulfide Oxidoreductase, Recombinant (dsbA)
Catalog No			USB-D3778-01
Supplier’s Catalog No			D3778-01
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			21.1

Storage			4°C/-20°C
Other names
Grade			Highly Purified
Purity			≥95% by SDS PAGE
Form			Supplied as a liquid in 20mM Tris pH 7.5, 2mM EDTA
Reactivity life			6 months
Note			For reserch purpose only
Purity			≥95% by SDS PAGE
Description			Recombinant Disulfide Oxidoreductase (rDsbA), produced from E. coli is a periplasmic protein and thioredoxin superfamily member which introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide(1-19 amino acids). Recombinant DsbA (residues 20-208) was expressed in E. coli and purified by using conventional chromatography techniques. Molecular Weight: 21.1kD (189 amino acids) Sequences: AQYEDGKQYT/ TLEKPVAGAP/ QVLEFFSFFC/ PHCYQFEEVL/ HISDNVKKKL/ PEGVKMTKYH/ VNFMGGDLGK/ DLTQAWAVAM/ ALGVEDKVTV/ PLFEGVQKTQ/ TIRSASDIRD/ VFINAGIKGE/ EYDAAWNSFV/ VKSLVAQQEK/ AAADVQLRGV/ PAMFVNGKYQ/ LNPQGMDTSN/ MDVFVQQYAD/ TVKYLSEKK Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.