RSPO-3, Recombinant, Mouse (R-spondin-3, Cabriolet, Cysteine-rich and Single Thrombospondin Domain-containing Protein 1, Cristin-1, Nucleopondin, Roof Plate-specific Spondin-3, Rspo3)
Catalog No : USB-R9417-52
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| Product name | RSPO-3, Recombinant, Mouse (R-spondin-3, Cabriolet, Cysteine-rich and Single Thrombospondin Domain-containing Protein 1, Cristin-1, Nucleopondin, Roof Plate-specific Spondin-3, Rspo3) | ||
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| Catalog No | USB-R9417-52 | ||
| Supplier’s Catalog No | R9417-52 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Chinese Hamster Ovary cell line | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 29 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDS-PAGE, reducing conditions) | ||
| Form | Supplied as a lyophilized powder from PBS, BSA. Reconstitute with sterile PBS. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDS-PAGE, reducing conditions) | ||
| Description | RSpondin 3 (RSPO3, roof platespecific spondin 3), also called cysteinerich and single thrombospondin domain containing1 (Cristin 1), is an ~31kD secreted protein that shares ~40% aa identity with the other three RSpondin family members. All are positive modulators of Wnt/betacatenin signaling, but each has a distinct expression pattern. Like other Rspondins, RSpondin 3 contains two adjacent cysteinerich furinlike domains (aa35135) with one potential Nglycosylation site (aa 36), followed by a thrombospondin (TSP1) motif (aa147207) and a region rich in basic residues (aa211269). Only the furinlike domains are needed for betacatenin stabilization. Within aa21209, mouse RSpondin 3 shares 93%, 97%, 96%, 95% and 91% aa identity with human, rat, equine, bovine and canine RSpondin 3, respectively. Potential isoforms of 217, 224 and 252 aa are divergent or truncated at the C terminus; the 252 aa form also lacks aa433 at the Nterminus. Mouse RSpondin 3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development. It is also essential for expression of the placentaspecific transcription factor, Gcm1. In the mouse embryo, RSpondin 3 is often expressed by or located near endothelial cells. It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs. RSpondins regulate Wnt/betacatenin by competing with the Wnt antagonist DKK1 for binding to the Wnt coreceptors LRP6 and Kremen, reducing their DKK1mediated internalization. Reports differ on whether RSpondins bind LRP6 directly. RSpondin 3 has also been identified as an oncogene. Source: Chinese Hamster Ovary cell line, CHO-derived mouse R-Spondin 3 protein, Met1-His277. SDS-PAGE: 37-43kD, reducing conditions Activity: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.25-1 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a. Endotoxin Level: ≤0.10EU/1ug (LAL) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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