RSPO3, Recombinant, Human (R-Spondin-3, PWTSR, THSD2, Roof Plate-Specific Spondin-3)
Catalog No : USB-220238
432.20€
0.00€
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| Product name | RSPO3, Recombinant, Human (R-Spondin-3, PWTSR, THSD2, Roof Plate-Specific Spondin-3) | ||
|---|---|---|---|
| Catalog No | USB-220238 | ||
| Supplier’s Catalog No | 220238 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, CHO cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 37 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDS-PAGE) | ||
| Form | Supplied as a lyophilized powder in 10mM sodium phosphate, pH 7.5, 150mM sodium chloride. Reconstitute with sterile ddH2O to 0.1-1mg/ml. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDS-PAGE) | ||
| Description | The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization and resulting accumulation of the intracellular signaling protein, beta-catenin. This activity effectively activates and increases the subsequent nuclear signaling of beta-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/beta-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations. Recombinant human R-Spondin-3 is a 26.9kD protein consisting of 240aa residues. Due to glycosylation, R-Spondin-3 migrates at an apparent molecular weight of ~37kD by SDS PAGE analysis under reducing conditions. Source: Recombinant protein corresponding to 240aa residues from human RSPO3, expressed in CHO cells. Biological Activity: Enhances BMP-2 mediated differentiation of MC3T3-E1 cells. Endotoxin: <0.1ng/ug of protein (<1EU/ug) AA Sequence: MHPNVSQGCQ GGCATCSDYN GCLSCKPRLF FALERIGMKQ IGVCLSSCPS GYYGTRYPDI NKCTKCKADC DTCFNKNFCT KCKSGFYLHL GKCLDNCPEG LEANNHTMEC VSIVHCEVSE WNPWSPCTKK GKTCGFKRGT ETRVREIIQH PSAKGNLCPP TNETRKCTVQ RKKCQKGERG KKGRERKRKK PNKGESKEAI PDSKSLESSK EIPEQRENKQ QQKKRKVQDK QKSVSVSTVH Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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