Imugex

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Categories: Biochemicals / Biochemicals

Product name			5X All-In-One RT MasterMix (with ExCellenCT Lysis Kit)
Catalog No			IGX-143
Supplier’s Catalog No			IGX-143
Supplier			ImuGeX
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			Store all components at -20C
Other names
Grade
Purity
Form
Reactivity life			12 months
Note			For reserch purpose only
Description			Extracting and preparing RNA templates directly from cultured cells to be reverse-transcribed into cDNA, with 5X All-In-One RT MasterMix. ImuGeX's ExCellenCT Lysis Kit is an unique and effective alternative method to provide RNA templates that are extracted and processed directly from cultured cells in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template to be reverse-transcribed into cDNA using the 5X All-In-One RT MasterMix. <br /><br />The 5X All-In-One RT MasterMix is a ready-to-use formulation of all the reagents necessary for first-strand cDNA synthesis, including ImuGeX's proprietary OneScript® Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a finely balanced ratio of Oligo (dT)s and Random Primers. Coupled together, this complete system provides the ultimate convenience in generating high-quality cDNA directly from 10-105 cultured cells, suitable for a wide range of downstream applications._x000D_ _x000D_ This product is only intended for quick RNA extraction from cultured cells for two-steps RT-PCR or two-steps qRT-PCR gene expression analysis._x000D_ This product is not suitable for typical RNA/cDNA pool preparation.