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Categories: Peptides & Proteins / Proteins

Product name			Topoisomerase I, DNA, Recombinant, Human (Scl-70)
Catalog No			USB-T8065-02
Supplier’s Catalog No			T8065-02
Supplier			US Biologicals
Source antigen			Recombinant, Sf9 insect cells
Reactivity
Cross reactivity
Applications
Molecular weight			91.58

Storage			-20°C
Other names
Grade			Highly Purified
Purity			≥95% (SDS-PAGE)
Form			Supplied as a liquid in 16mM HEPES, pH 7.5, 400mM sodium chloride, 20% glycerol
Reactivity life			6 months
Note			For reserch purpose only
Purity			≥95% (SDS-PAGE)
Description			DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis. In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100 kD molecular weight. Most of this precursor is then proteolytically processed to a species with 70 kD molecular weight from which the Scl-70 antigen has derived its name. Recombinant Human Scl-70 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 dalton. Recombinant Scl-70 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.