Topoisomerase I, Recombinant, Human, His-Tag (DNA Topoisomerase-I, TOP1, TOP-I, Topoisomerase (DNA) I, Topoisomerase 1, Topoisomerase1, Topoisomerase-1, Topoisomerase-I, Type I DNA Topoisomerase)
Catalog No : USB-209247
417.25€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Product name | Topoisomerase I, Recombinant, Human, His-Tag (DNA Topoisomerase-I, TOP1, TOP-I, Topoisomerase (DNA) I, Topoisomerase 1, Topoisomerase1, Topoisomerase-1, Topoisomerase-I, Type I DNA Topoisomerase) | ||
|---|---|---|---|
| Catalog No | USB-209247 | ||
| Supplier’s Catalog No | 209247 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, sf9 insect cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 91.57 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | ~80% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 16mM HEPES, pH 8.0, 250mM sodium chloride, 20% glycerol. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~80% (SDS-PAGE) | ||
| Description | DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis. In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100kD molecular weight. Most of this precursor is then proteolytically processed to a species with 70kD molecular weight from which the Scl-70 antigen has derived its name. Recombinant protein corresponding to human TOP1, fused with a 6xHis-tag, expressed in Sf9 insect cells. Molecular Weight: ~91.57kD Specificity: Binds IgG-type human auto-antibodies. Applications: Suitable for use in ELISA, Western Blot, Biotinylation and Iodination. Other applications not tested. Recommended Dilution: ELISA: 0.5-0.8ug/ml, (depending on the type of ELISA plate and coating buffer). Standard ELISA test: (checker-board analysis of positive/negative sera panels including CDC international reference sera). Western Blot: with monoclonal anti-hexa-His-tag antibody and Scleroderma patient sera. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
Product Details
© 2020 Imugex All Rights Reserved