NFkB, p50, Recombinant, Human, GST-Tag (delta434-969) (NF-kB, Nuclear Factor kappa B p50 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B cells 1, NFkB1, DKFZp686C01211, DNA Binding Factor KBF1, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kB, Nuclear Factor kappa B DNA Binding Subunit)
Catalog No : USB-N2301-52G
693.12€
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| Product name | NFkB, p50, Recombinant, Human, GST-Tag (delta434-969) (NF-kB, Nuclear Factor kappa B p50 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B cells 1, NFkB1, DKFZp686C01211, DNA Binding Factor KBF1, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kB, Nuclear Factor kappa B DNA Binding Subunit) | ||
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| Catalog No | USB-N2301-52G | ||
| Supplier’s Catalog No | N2301-52G | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 74 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | Purified by Glutathione agarose chromatography. ≥ 90%, determined by SDS-PAGE and Coomassie blue staining. | ||
| Form | Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.1% beta-mercaptoethanol, 0.03% Brij-35, 0.2mM PMSF, 1mM benzamidine and 50% glycerol. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified by Glutathione agarose chromatography. ≥ 90%, determined by SDS-PAGE and Coomassie blue staining. | ||
| Description | This gene encodes a 105kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50kD protein. The 105kD protein is a Rel protein-specific transcription inhibitor and the 50kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Source: N-terminal GST-tagged, recombinant human NFkB, p50 subunit (delta434-969), expressed in E. coli. Quality Control Testing: MS Tryptic Fingerprint: Confirmed identity as NFkB p50 subunit with 35% amino acid coverage of the translated native sequence DNA Binding Assay: The activity of GST-NFkB p50 subunit was assessed by its ability to bind to 32P labelled DNA probes H2-kB or HIV-kB in an electrophoretic mobility shift assay. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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