GTF2B 1-316aa, Recombinant Human, His-Tag (General Transcription Factor IIB, TF2B, TFIIB)

Catalog No : USB-G2024-61H
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Product name GTF2B 1-316aa, Recombinant Human, His-Tag (General Transcription Factor IIB, TF2B, TFIIB)
Catalog No USB-G2024-61H
Supplier’s Catalog No G2024-61H
Supplier US Biologicals
Source antigen Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight 36.9
Storage -20°C/-70°C
Other names
Grade Affinity Purified
Purity ~90% (SDS-PAGE) Purified by immunoaffinity chromatography.
Form Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol, 0.1M sodium chloride.
Reactivity life 6 months
Note For reserch purpose only
Purity ~90% (SDS-PAGE) Purified by immunoaffinity chromatography.
Description GTF2B, also known as transcription factor II B, is one of several general transcription factors that make up the RNA polymerase II preinitiation complex. It localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. The protein serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. It is involved in the selection of the transcription start site. Recombinant GTF2B protein was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant GTF2B protein was expressed in E. coli AA sequence: MGSSHHHHHH SSGLVPRGSH MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF PTDFKFDTPV DKLPQL Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.