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Categories: Peptides & Proteins / Proteins

Product name			Lin28, Recombinant, Human, His-Tag, aa42-209
Catalog No			USB-L2280-49
Supplier’s Catalog No			L2280-49
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			21.1

Storage			-20°C
Other names
Grade			Purified
Purity			~80% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 0.1mM PMSF, 10% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~80% (SDS-PAGE)
Description			Lin-28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. It is marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. It has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Recombinant Lin28 protein was expressed in E. coli and purified by using conventional chromatography techniques. Recombinant protein corresponding to human Lin28, his-tagged, expressed in E. coli. Accession: NP_078950 AA sequence: MGSSHHHHHH SSGLVPRGSH RSMGICKWFN VRMGFGFLSM TARAGVALDP PVDVFVHQSK LHMEGFRSLK EGEAVEFTFK KSAKGLESIR VTGPGGVFCI GSERRPKGKS MQKRRSKGDR CYNCGGLDHH AKECKLPPQP KKCHFCQSIS HMVASCPLKA QQGPSAQGKP TYFREEEEEI HSPTLLPEAQ N Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.