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Categories: Biochemicals / Biochemicals

Product name			MTT Cell Proliferation and Cytotoxicity Assay Kit
Catalog No			BAR1005
Supplier’s Catalog No			BAR1005
Supplier			Biospes
Source antigen			N/A
Reactivity			Cells
Cross reactivity			None
Applications			Cell proliferation and cytotoxicity assay
Molecular weight			N/A

Storage			MTT Staining Solution Store at -20°C in dark for one year; Formazan diluent buffer Store at 2-8°C for one year.
Other names
Grade			Molecular biology
Purity			High
Form			Liquid
Reactivity life			12 months
Note			For reserch purpose only
Description			1. Since cell viability and numbers were positively correlated, MTT Cell Proliferation and Cytotoxicity Assay Kit was used widely to detect the cell proliferation and cytotoxicity. MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide] can be reverted to the purple crystal Formazan by the dehydrogenase of mitochondria, and the Formazan can be solved thoroughly by a certain solvent. Read the O.D. Value at 570 nm, the O.D. Value is more higher, the cell proliferation is more sooner; conversely, if the O.D. Value is more lower, the cell cytotoxicity is more bigger. 2. With the unique Formazan diluent buffer, it is not necessary to remove the intrinsic cell culture medium, but add the kit component Formazan diluent buffer directly, in this way, the unnecessary assay error can be avoided. 3. With the advantages of low background, high sensitivity, easy operation and wide linearity range, this kit can increase the reliability of the assay.