POLR2J3, Recombinant, Human, aa1-115, His-Tag (DNA-directed RNA Polymerase II Subunit RPB11-b2, POLR2J2, RPB11b1, RPB11b2)

Catalog No : USB-138716
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Product name POLR2J3, Recombinant, Human, aa1-115, His-Tag (DNA-directed RNA Polymerase II Subunit RPB11-b2, POLR2J2, RPB11b1, RPB11b2)
Catalog No USB-138716
Supplier’s Catalog No 138716
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 15.5
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride,1mM DTT, 40% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Source: Recombinant protein corresponding to aa1-115 from human POLR2J3 at N-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~15.5kD (138aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGS HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP MNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.