NFkB, p50, Human, Recombinant, His-Tag (Nuclear Factor NF-kappaB p50 Subunit)
Catalog No : USB-207299
548.29€
0.00€
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| Product name | NFkB, p50, Human, Recombinant, His-Tag (Nuclear Factor NF-kappaB p50 Subunit) | ||
|---|---|---|---|
| Catalog No | USB-207299 | ||
| Supplier’s Catalog No | 207299 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Sf21 cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥95% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 50mM TRIS-HCl, pH 7.5, 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole, 10% glycerol. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥95% (SDS-PAGE) | ||
| Description | NF-kappaB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric p65-p50 complex is the most abundant complex. The dimers bind at kB sites in the DNA of their target genes and the individual dimers have distinct preferences for different kB sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kB complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kB inhibitor (I-kB) family. In a conventional activation pathway, I-kB is phosphorylated by I-kB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kB complex which translocates to the nucleus. NF-kB heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kB p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. Source: Recombinant protein corresponding to human NF-KappaB, fused to His-tag, expressed in Sf21 cells. Biological Activity: ~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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