Nuclear and Cytoplasmic Protein Extraction Kit

Catalog No : BWR1008
286.90€
0.00€

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Product name Nuclear and Cytoplasmic Protein Extraction Kit
Catalog No BWR1008
Supplier’s Catalog No BWR1008
Supplier Biospes
Source antigen N/A
Reactivity Eukaryotic samples,: cultured cells and fresh tissues.
Cross reactivity No cross reactivity
Applications WB
Molecular weight N/A
Storage Store at -20°C
Other names
Grade Molecular biology
Purity High
Form Liquid
Reactivity life 12 months when stored properly
Note For reserch purpose only
Description 1. Nuclear and Cytoplasmic Protein Extraction Kit is designed to quickly prepare highly enriched fractions of cytoplasmic and nuclear proteins from eukaryotic samples, such as cultured cells and fresh tissues. 2. This kit provides a simple and convenient method for extracting nuclear and cytoplasmic protein. The user can complete the separation of nuclear and cytoplasmic protein within about 90 min. Extracted proteins can be directly apply for downstream reactions, such as Western, EMSA, foot printing, reporter gene detection, and measurement of enzyme activity. 3. Through Cytoplasmic Protein Extraction Reagent A and B, this kit breaks cell membrane and releases proteins for cell burst under hypotonic condition, then centrifuge for collection of the nucleoli. At last, extract the nuclear proteins by hyperhaline Nuclear Protein Extraction Reagent. 4. The kit can be used for 50 samples (60 mg / sample, or 2 ×106 Hela cells). User can adjust the volume of reagent according to different sample volumes.