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Categories: Biochemicals / Biochemicals

Product name			Cell Mitochondria Isolation Kit
Catalog No			BWR1010
Supplier’s Catalog No			BWR1010
Supplier			Biospes
Source antigen			N/A
Reactivity			Mitochondria
Cross reactivity			No cross reactivity
Applications			WB, IP, Citrate synthase activity, Cytochrome c oxidase activity
Molecular weight			N/A

Storage			Store at -20°C
Other names
Grade			Molecular biology
Purity			High
Form			Liquid
Reactivity life			12 months when stored properly
Note			For reserch purpose only
Description			1. Besides the mitochondria, this kit can obtain the mitochondria-free cytoplasmic protein which can be used for studying the release of mitochondrial protein and cytoplasm. 2. This kit enables the fast and easy isolation of an enriched and pure mitochondrial fraction from cells. And most of the isolated mitochondria will contain intact inner and outer membranes. 3. Lysed by the Mitochondria Lysis Buffer (kit component), the isolated mitochondria can be used for SDS-PAGE, Western blot and Di-electrophoresis assays. 4. This kit provides the Trypan Blue Staining Buffer (optional kit component) for controlling homogenization, this can help to obtain more intact mitochondria. 5. The kit component-PMSF as protease inhibitor can quench the protease activity properly during homogenization. 6. This kit is sufficient for 50-100 applications (2-5 × 107), isolation of enriched mitochondrial fraction from cells.