ATF1, Recombinant, Human, aa1-271, His-Tag (Activating transcription factor 1, EWS-ATF1, FUS/ATF-1, TREB36)

Catalog No : USB-351094
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Product name ATF1, Recombinant, Human, aa1-271, His-Tag (Activating transcription factor 1, EWS-ATF1, FUS/ATF-1, TREB36)
Catalog No USB-351094
Supplier’s Catalog No 351094
Supplier US Biologicals
Source antigen Recombinant, human from E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 31.8
Storage -20°C/-70°C
Other names
Grade Purified
Purity ~90% (SDS-PAGE)
Form Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 5mM DTT, 0.2M sodium chloride, 2mM EDTA, 50% glycerol.
Reactivity life 12 months
Note For reserch purpose only
Purity ~90% (SDS-PAGE)
Description Activating transcription factor 1, also known as ATF1, is a cyclic-AMP dependent transcription factor. ATF1 is expressed in a wide variety of cell types and is capable of dimerizing with CREB. MSK1 and MSK2 protein kinases are required for the stress-induced phosphorylation of transcription factors CREB and ATF1 in primary embryonic fibroblasts. Epidermal growth factor induction of c-jun expression requires ATF1 and MEF2 sites in the c- jun promoter Source: Recombinant protein corresponding to aa1-271 from human ATF1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~31.8kD (295aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEDSHK STTSETAPQP GSAVQGAHIS HIAQQVSSLS ESEESQDSSD SIGSSQKAHG ILARRPSYRK ILKDLSSEDT RGRKGDGENS GVSAAVTSMS VPTPIYQTSS GQYIAIAPNG ALQLASPGTD GVQGLQTLTM TNSGSTQQGT TILQYAQTSD GQQILVPSNQ VVVQTASGDM QTYQIRTTPS ATSLPQTVVM TSPVTLTSQT TKTDDPQLKR EIRLMKNREA ARECRRKKKE YVKCLENRVA VLENQNKTLI EELKTLKDLY SNKSV Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.