NME2, Recombinant, Human, aa1-152 (Nucleoside diphosphate Kinase B, NDPK-B, NDPKB, NM23-H2, NM23B)
Catalog No : USB-351393
411.51€
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| Product name | NME2, Recombinant, Human, aa1-152 (Nucleoside diphosphate Kinase B, NDPK-B, NDPKB, NM23-H2, NM23B) | ||
|---|---|---|---|
| Catalog No | USB-351393 | ||
| Supplier’s Catalog No | 351393 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, human from E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 17.2 | ||
| Storage | -20°C/-70°C | ||
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| Other names | |||
| Grade | Purified | ||
| Purity | ~90% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~90% (SDS-PAGE) | ||
| Description | NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Source: Recombinant protein corresponding to aa1-152 from human NME2, expressed in E. coli. Molecular Weight: ~17.2kD (152aa) Specific Activity: >1800units/mg, and is defined as the amount of enzyme that convert 1umole each of ATP and TDP to ADP and TTP /minute at pH 7.5 at 25°C in a couple system with PK/LDH. AA Sequence: MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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