ANAPC2, GST-Tag, Recombinant (APC2, RP11-350O14.5, Anaphase-Promoting Complex Subunit 2)

Catalog No : USB-500595
482.77€
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Product name ANAPC2, GST-Tag, Recombinant (APC2, RP11-350O14.5, Anaphase-Promoting Complex Subunit 2)
Catalog No USB-500595
Supplier’s Catalog No 500595
Supplier US Biologicals
Source antigen E. coli, Recombinant
Reactivity
Cross reactivity
Applications
Molecular weight 59
Storage -20°C
Other names
Grade Lysate
Purity Lysate
Form Supplied as a lyophilized powder. Reconstitute with 200ul sterile dH2O.
Reactivity life 6 months
Note For reserch purpose only
Purity Lysate
Description A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. Source: Partial length protein corresponding to of ANAPC2, fused to GST-Tag at N-terminal, expressed in E. coli. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Molecular Weight: ~59kD Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.