FOXP3 (FOXP3, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, SCURFIN, scurfin, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked) (Control Peptide)
Catalog No : USB-F9050-10A-P
421.85€
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| Product name | FOXP3 (FOXP3, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, SCURFIN, scurfin, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked) (Control Peptide) | ||
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| Catalog No | USB-F9050-10A-P | ||
| Supplier’s Catalog No | F9050-10A-P | ||
| Supplier | US Biologicals | ||
| Source antigen | Synthetic | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly Purified | ||
| Form | Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly Purified | ||
| Description | SQRPSRCSNPTPGP, C Terminus of human FOXP3. Control peptide corresponding to polyclonal antibody, Catalog#F9050-10A, goat x human. FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buf Peptide Blocking (see corresponding antibody F9050-10A): Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. | ||
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