Imugex

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Categories: Peptides & Proteins / Proteins

Product name			RCHY1, Recombinant, Human, aa1-261, His-Tag (RING Finger and CHY Zinc Finger Domain-containing Protein 1, ARNIP, CHIMP, hARNIP, PIRH2, PIRH2E, PIRH2F, PRO1996, RNF199, ZNF363)
Catalog No			USB-137182
Supplier’s Catalog No			137182
Supplier			US Biologicals
Source antigen			Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight			32.5

Storage			-20°C
Other names
Grade			Purified
Purity			~85% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.4M urea, 10% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~85% (SDS-PAGE)
Description			RCHY1 has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including TP53, HDAC1 and CDKN1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Source: Recombinant protein corresponding to human RCHY1, fused to His-tag at N-terminal expressed in E.coli. Molecular Weight: ~32.5kD (284aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAATARE DGASGQERGQ RGCEHYDRGC LLKAPCCDKL YTCRLCHDNN EDHQLDRFKV KEVQCINCEK IQHAQQTCEE CSTLFGEYYC DICHLFDKDK KQYHCENCGI CRIGPKEDFF HCLKCNLCLA MNLQGRHKCI ENVSRQNCPI CLEDIHTSRV VAHVLPCGHL LHRTCYEEML KEGYRCPLCM HSALDMTRYW RQLDDEVAQT PMPSEYQNMT VDILCNDCNG RSTVQFHILG MKCKICESYN TAQAGGRRIS LDQQ Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.